Aerobic methanotrophic bacterial communities in sediments of Lake Constance
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چکیده
25 Introduction 26 Materials and Methods 28 Results 36 Discussion 47 3. Characterization of a novel methanotroph, Methylosoma difficile gen. nov., sp. nov., enriched by gradient cultivation from littoral sediment of Lake Constance 55 Summary 55 Introduction 57 Methods 58 Results and 64 Discussion 4. Comparison of aerobic methanotrophic communities in littoral and profundal sediment of Lake Constance by a molecular approach 74 Abstract 74 Introduction 75 Materials and Methods 76 Results 78 Discussion 8274 Introduction 75 Materials and Methods 76 Results 78 Discussion 82 5. Depth distribution of methanotrophic bacteria in littoral and profundal sediments of Lake Constance 85 Abstract 85 Introduction 86 Materials and Methods 88 Results 94 Discussion 10385 Introduction 86 Materials and Methods 88 Results 94 Discussion 103 6. Oxygen Relationships and Ultra-structure of a novel methanotroph 112 Abstract 112 Introduction 113 Materials and Methods 115 Results 118 Discussion 126 7. General Discussion 134
منابع مشابه
Comparison of aerobic methanotrophic communities in littoral and profundal sediments of Lake Constance by a molecular approach.
Analysis of pmoA and 16S rRNA gene clone libraries of methanotrophic bacteria in Lake Constance revealed an overall dominance of type I methanotrophs in both littoral and profundal sediments. The sediments exhibited minor differences in their methanotrophic community structures. Type X methanotrophs made up a significant part of the clone libraries only in the profundal sediment and were also f...
متن کاملActivity and diversity of methanotrophic bacteria at methane seeps in eastern Lake Constance sediments.
The activity and community structure of aerobic methanotrophic communities were investigated at methane seeps (pockmarks) in the littoral and profundal zones of an oligotrophic freshwater lake (Lake Constance, Germany). Measurements of potential methane oxidation rates showed that sediments inside littoral pockmarks are hot spots of methane oxidation. Potential methane oxidation rates at littor...
متن کاملAerobic and Anaerobic Oxidation of Methane in Sediments of Lake Constance
13 Introduction 13 Materials and Methods 15 Results 19 Discussion 23 Acknowledgements 29 Chapter 3 Activity and Diversity of Methanotrophic Bacteria at Methane Seeps in Eastern Lake Constance Sediments 30 Abstract 30 Introduction 30 Materials and methods 31 Results 35 Discussion 42 Acknowledgements 4530 Introduction 30 Materials and methods 31 Results 35 Discussion 42 Acknowledgements 45
متن کاملpmoA-based analysis of methanotrophs in a littoral lake sediment reveals a diverse and stable community in a dynamic environment.
Diversity and community structure of aerobic methane-oxidizing bacteria in the littoral sediment of Lake Constance was investigated by cloning analysis and terminal restriction fragment length polymorphism (T-RFLP) fingerprinting of the pmoA gene. Phylogenetic analysis revealed a high diversity of type I and type II methanotrophs in the oxygenated uppermost centimeter of the sediment. T-RFLP pr...
متن کاملMethane release from sediment seeps to the atmosphere is counteracted by highly active Methylococcaceae in the water column of deep oligotrophic Lake Constance.
Methane emissions from freshwater environments contribute substantially to global warming but are under strong control of aerobic methane-oxidizing bacteria. Recently discovered methane seeps (pockmarks) in freshwater lake sediments have the potential to bypass this control by their strong outgassing activity. Whether this is counteracted by pelagic methanotrophs is not well understood yet. We ...
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